H&E Analysis

ViraPath H&E Analysis detects epithelial regions, cells of the lumen and tumor in the tissues stained with Hematoxylin and Eosin (H&E). The areas of tumor cell nuclei and all the tumoral regions are calculated. Tumor cell nuclei are classified according to the pleomorphism criterion of Nottingham Histologic Grade.

Nottingham Histological Grade:

1) Glandular(Acinar)/Tubular Differentiation   

  • Score 1:  >75% of tumor area forming glandular/tubular structures
  • Score 2: 10% to 75% of tumor area forming glandular/tubular structures
  • Score 3:  <10% of tumor area forming glandular/tubular structures

2) Nuclear Pleomorphism

  • Score 1: Uniform cells with small nuclei similar in size to normal breast cells 
  • Score 2: Moderate variability in nuclear size and shape
  • Score 3: Marked variation in nuclear size and shape

3) Mitotic Count

  • Score 1: 0-9 mitoses per 10 high power fields
  • Score 2: 10-19 mitoses per 10 high power fields 
  • Score 3: > 20 mitoses per 10 high power fields 

Tubule formation, nuclear pleomorphism, and mitotic count are separately evaluated between 1 and 3. Their sum reaches a total score ranging from 3 to 9.

Score 3 to 5: Grade 1

Score 6 to 7: Grade 2

Score 8 to 9: Grade 3

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